Abstract: A alginate-producing lyase bacterial strain AlgX2 was selected in the viscera of starfish Asteroidea sp. using sodium alginate as sole carbon source in order to screen biodegradable alginate lyase microbial strain which was identified to belong to the genus of Cobetia, named as Cobetia AlgX2, upon on the identification of strain morphology, physiology, biochemistry and 16S rRNA gene. The enzyme activity of the strain determined by the DNS method was used as an indicator, and the culture medium and conditions of the stain were optimized by orthogonal experiment. The results showed that the optimum enzyme-producing medium for the stain was observed under conditions of 14 g/L of alginate, 4 g/L of ammonium sulfate, 30 g/L of NaCl, initial pH of 6.0, culture temperature of 22 ℃, inoculation volume of 2%, liquid volume of 50 mL/250 mL and speed of 150 r/min. Under the optimal conditions, an enlarged volume of 10 L fermentation was applied in culturing and producing the enzyme, with the enzyme activity of 0.176 U/mL. HPLC analysis of alginate oligosaccharides prepared by enzymatic hydrolysis of crude enzyme liquid revealed that the samples had the polymerization degree of dp2-dp5. The findings indicate that this strain can be used to expand fermentation production, high enzyme yielding efficiency and significant alginate cracking effect.
国晶晶, 宋悦凡, 何云海, 武龙, 汪秋宽. 高效产褐藻胶裂解酶菌株产酶条件优化及降解寡糖结构分析[J]. 大连海洋大学学报, 2019, 34(2): 252-259.
GUO Jing-jing, SONG Yue-fan, HE Yun-hai, WU Long, WANG Qiu-kuan. Optimization of enzyme production conditions and analysis of structure of degraded oligosaccharides in high-yield alginate lyase bacterial strains. Journal of Dalian Ocean University, 2019, 34(2): 252-259.
2019, Vol. 34 
