Abstract: Naupli of brine shrimpArtemiasalina was reared in aquaculture wastewater at density of 0 (control group), 100, 200, and 400 individuals/L for 7 days and then co-cultured with green algaChlorella sp. for 17 days in a laboratory to explore brine shrimp- green alga combined treatment effect on removal of suspended substance(SS), ammonia nitrogen and total phosphorus in aquaculture wastewater. The SS content was found to be to the minimum in aquaculture wastewater 7 days after treatment, with significant difference between each experimental group and the control group (P<0.05). The maximal removal rate (93.53%) of ammonia nitrogen was observed in the 400 ind./L group, and the maximal removal rate of total phosphorus(91.39%) at 200 ind./L group in 17 day treatment, with significant difference in body length of the brine shrimp between brine shrimp- green alga combined treatment group and the control group(P<0.05). The removal rates of ammonia nitrogen and total phosphorus were as high as 90% from aquaculture wastewater in brine shrimp- green alga combined treatment, with the best removal effects of ammonia nitrogen and total phosphorus in 200 ind./L group. The findings indicate that brine shrimp- green alga combined treatment of aquaculture effluent not only provides live food for aquaculture to reduce costs, but also deals with aquaculture wastewater as a new effective and practical way.