Interactive effect of dietary leucine and isoleucine on digestive and immune-related enzyme activities of Japanese flounder Paralichthys olivaceus
WANG Li-ping,JIANG Zhi-qiang,SUN Meng-lei,SIBin,HAN Yu-zhe,CHEN Fei,BAO Ning
College of Fisheries and Life Science,Key Laboratory ofMariculture&Stock Enhancement in North China蒺s Sea,Ministry of Agriculture,Key Laborato-ry of Fish Applied Biology and Aquaculture in North China,Dalian Ocean University,Dalian 116023,China
Abstract: A feeding trailwas conducted to investigate the interactive effectof dietary leucine(Leu)and isoleucine (Ile)on activities of intestine digestive enzymes and several liver immune-related enzymes of juvenile Japanese flounder Paralichthysolivaceus.Japanese flounder juvenileswith initialweights of(2.69依0.04)gwere fed six diets (LL-LI,LL-MI,LL-HI,HL-LI,HL-MI,HL-HI)containing two levels of dietary Leu(2.58%and 5.08%in drymatter)combined with three levels of dietary Ile(1.44%,2.21%and 4.44%in drymatter)with triplicate tanks per treatment for 60 days,and then intestinal digestive enzymes(protease and Lipase)and liver immune-re-lated enzymes including catalase(CAT),superoxide dismutase(SOD),acid phosphatase(ACP),and alkaline phosphatase(AKP)activities and total antioxidant capacity(T-AOC)weremeasured in the test fish.The activi-ties of intestinal digestive enzymes in high dietary Ile were shown significantly higher than middle Ile groups(P< 0.05)and it can be decreased in high dietary Leu(except LI).In low Leu groups,the activities of liver CAT, SOD and ACP aswell as AKP was significantly increased(P<0.05)with the increasing dietary Ile at dietary Ile level逸2.21%.In high Leu groups,however,the activities of CAT,SOD and AKPwere found to be significantly decreased(P<0.05)with the increase in dietary Ile level at dietary Ile level逸2.21%.It can be concluded that significant interactive effect of dietary Leu and Ile is found in intestine digestive enzyme activities as well as liver anti-oxidant enzyme and enzyme related to immunity activities of Japanese flounder.The higher digestive and im-mune-related enzyme activitieswere observed in Japanese flounder fed diet containing low Leu(2.58%)and high Ile(4.44%).
王莉苹, 姜志强, 孙梦蕾, 司滨, 韩雨哲, 陈飞, 暴宁. 饲料中亮氨酸和异亮氨酸交互作用对牙鲆消化酶及免疫相关酶活力的影响[J]. 大连海洋大学学报, 2017, 32(3): 287-293.
WANG Li-ping, JIANG Zhi-qiang, SUN Meng-lei, SIBin, HAN Yu-zhe, CHEN Fei, BAO Ning. Interactive effect of dietary leucine and isoleucine on digestive and immune-related enzyme activities of Japanese flounder Paralichthys olivaceus. Journal of Dalian Ocean University, 2017, 32(3): 287-293.
2017, Vol. 32 
