1.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;2.Dalian Fisheries Industry Technology Innovation Associ-ation,Dalian 116023,China
Abstract: Tissue expression levels of four candidate genes including tenascin gene,collagen琢2 gene,integrin琢V gene and integrin茁L gene were investigated in differentmonth old sea cucumber Apostichopus japonicus by RTPCT.The results showed that therewere different expression levels of tenascin gene in intestines,tube foot,respir-atory tree,skin,body wall,coelomic fluid,parapodium and longitudinalmuscle of the sea cucumber at age of12, 13,and 14 months,significantly higher in coelomic fluid and respiratory tree than in other tissues(P<0.05).The expression levels of collagen琢2 gene were shown to be increased in tube foot,respiratory tree,body wall,parapo-dium and longitudinalmuscle with increase in month age,themaximal expression in respiratory tree,and gradual decline in longitudinalmuscle,parapodium,body wall,intestines and coelomic fluid.The expression levels of in-tegrin琢V gene were found to be gradually increased in longitudinalmuscle,parapodium and body wallwithmonth age increasing.There were lower expression levels of integrin茁L gene in skin,body wall,parapodium and tube foot.The findings provided foundation for further understanding of the four genes in sea cucumber.
2017, Vol. 32 
