Abstract: To exploit recomibinant protein of tiger pufferfish Takifugu rubripes by expression of immune gene and recominant immune factors,the tiger pufferfish interleukin-15（IL15） gene sequence was obtained from kidney tissue by RT-PCR,and the recombinant DNA p ET-32a（＋）-IL15 was constructed by link mature peptide gene sequences and prokaryotic expression vector p ET-32a（＋）.The genetic engineering bacteria of recombinant expression IL15 by transforming p ET-32a（＋）-IL15 into Escheria coli BL21（DE3）,and the fusion proteins were obtained under the induction of IPTG.The purification of the express products and Western Blotting test revealed that there was high-efficiency expression of recombinant DNA p ET-32a（＋）-IL15 in E.coli with the accurate target protein,and fusion protein concentration of 294.6 μg / m L.The evaluation of fusion protein activity by MTT method showed that the CTLL-2 cell proliferation rate was 71% and 73% at the diluted 10 times and 100 times fusion protein,respectively.The findings indicate that the recombinant IL15 fusion protein has the ability to promote the proliferation of CTLL-2 cells in vitro.
孙赛红, 马普, 李慧, 孙鹤, 孔德荣, 李凤华, 仇雪梅, 姜志强, 刘海映. 红鳍东方鲀白介素15的原核表达及其活性测定[J]. 大连海洋大学学报, 2015, 30(6): 598-603.
SUN Sai-hong, MA Pu, LI Hui, SUN He, KONG De-rong, LI Feng-hua, QIU Xue-mei, JIANG Zhi-qiang, LIU Hai-ying, LIU Sheng-chong, WANG Xiu-li. Prokaryotic expression and biological activity evaluation of recombinan IL15 from tiger pufferfish Takifugu rubripes. Journal of Dalian Ocean University, 2015, 30(6): 598-603.
2015, Vol. 30 
