1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Abstract: In this study,RNA extract in liver of taimen Hucho taimen was used as a template,and IGF-Ⅱ gene open reading frame was amplified by one step RT-PCR using primers designed by Atlantic salmon IGF-Ⅱ gene indexed by Gen Bank（NCBI No.NM_ 001123647） to evaluate role of fish insulin-like growth factor-Ⅱ（IGF-Ⅱ） in the development of taimen embryos.Bioinformatics analysis revealed that the IGF-Ⅱ contained an ORF of 645 bp encoding 214 amino acid with a signal peptides and five regions named as B,C,A,D,and E.The IGF-Ⅱ of taimen had molecular weight of 24 554 and the isoelectric point of 9.92,shared high identity（98.6%） with salmon and the homology of the IGF-Ⅱ gene at nucleotide and amino acid level.The IGF-ⅡmRNA expression was detected in different tissues using Real-Time quantitative PCR technique.It was found that the maximal expression was observed in the liver,followed by in the heart,gill,spleen,hindgut,kidney,stomach,and muscle,and the minimal expression in the foregut and brain.
2015, Vol. 30 
