Abstract: Microsatellite loci were isolated and characterized through the magnetic beads enrichment method, and polymorphism evaluation and genetic differences were investigated in two wild populations(Qingdao, QD and Yangtze River Delta, CJK) of golden cuttlefish Sepia esculenta along coastal waters in China to explore the genetic diversity of the species. The population genetic evaluation of microsatellite markers indicated that in 269 clones 192 clones contained microsatellite sequences(71.38%), and 85 pairs of primers were designed based on these sequences, among which 20 pairs were clearly amplified and shown to be polymorphic, perfect SSR(accounting for 60%), imperfect SSR(10%), and compound SSR(30%). The numbers of alleles per locus were ranged from 2 to 17(average 8.15), the observed from 0.146 to 0.936(average 0.630)and expected heterozygosities from 0.172 to 0.930(mean 0.702). Fifteen of twenty loci was conformed to Hardy-Weinberg equilibrium and no significant linkage disequilibrium was found between any pair of loci after Bonferroni correction. Six and four loci displayed successful cross-species amplification in two other closely related species, Sepia andreana and Sepiella maindroni. A total of 163 alleles were detected in QD population, and 152 alleles in CJK population in 11 polymorphic loci analysis of population genetic diversity. The number of alleles was ranged from 5 to 28, with average allele number of 14.818, and 6 to 26, with average allele number of 13.818. There were 19 unique alleles in QD population,with observed heterozygosity from 0.250 to 0.936, with an average of 0.731, expected heterozygosity from 0.265 to 0.930 with an average of 0.771, polymorphic information content(PIC) from 0.223 to 0.946 with an average 0.746. There were 8 unique alleles in CJK population, with observation heterozygosity from 0.500 to 0.896(average 0.731), expected heterozygosity from 0.623 to 0.960(average 0.857), and PIC from 0.614 to 0.948 with an average 0.845. The genetic differentiation value(Fst) of 0.032 5 was
王鹤, 李战军, 王亮, 王增东, 贺加贝, 刘永胜. 金乌贼微卫星标记开发及两个野生群体遗传多样性比较[J]. 大连海洋大学学报, 2019, 34(4): 482-491.
WANG He, LI Zhan-jun, WANG Liang, WANG Zeng-dong, HE Jia-bei, LIU Yong-sheng. Isolation of microsatellite markers and genetic diversity of wild populations in golden cuttlefish Sepia esculenta. Journal of Dalian Ocean University, 2019, 34(4): 482-491.
2019, Vol. 34 
