DUAN Jing1, WU Li-fang1*, WANG Jing-yao1, ZHANG Dong-ming1, ZU Xiu-jie2, LIU Yan-hui2, JIN Chang-zhu3
1. College of Animal Science and Technology, Key Laboratory of Animal Production, Product Quality and Security, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China; 2.Fisheries Science Research Institute of Jilin Province, Changchun 130033, China; 3.Jinyuan Aquatic Products Field of Changyi District Jilin City, Jilin 132001, China
Abstract: A 56-day feeding trial was conducted to evaluate the effects of replacing fish meal with different soybean proteins on growth performance, non-specific immune indices and protein metabolism of fish Rhynchocyprislagowskii Dybowski with initial body weight of (6.09±0.03) g. The fish juveniles were reared in a temperature control culture system and fed five isonitrogen and isoenergic diets containing normal amount of fish meal (control group), soybean meal(SBM group), fermented soybean meal (FSBM group), full fat soybean meal(FFSBM group), and expanded soybean powder(ESBM group) replacing 50% of fish meal with 3 replicates with 40 fish in each treatment at water temperature of 22-24 ℃. It was found that there were significantly lower final body weight(FBW), weight gain rate(WGR), and specific growth rate(SGR) in the fish in SBM group, FSBM group, FFSBM group, and ESBM group than those in the control group(P<0.05). However, FBW, WGR, and SGR were significantly higher in FSBM group, and ESBM group than those in SBM group and FFSBM group(P<0.05). The juveniles in FSBM group had crude protein content in muscles, without significantly different from that in the control group (P>0.05), while the fish juveniles in SBM, FFSBM and ESBM groups had significantly lower muscular crude protein content than the fish in the control group did (P<0.05). There was significantly lower activity of lysozyme (LZM) in SBM, FSBM, FFSBM and ESBM groups than that in the control group (P<0.05), without significant difference between FSBM and ESBM groups(P>0.05), and significantly higher than that in SBM and the FFSBM groups(P<0.05). Significantly lower protease activities in hepatopancreas, foregut, and hindgut were observed in the fish in SBM, FSBM, FFSBM, and ESBM groups than that in the control group (P<0.05). Protease activity of middle intestine were significantly lower in SBM and FFSBM groups than that in the control group (P<0.05). Glutamic oxaloacetic transaminase GOT and glutamic-pyruvic transaminase (GPT) activities were found to be significantly lower in SBM, FSBM, FFSBM, and ESBM groups than those in the control group (P<0.05), significantly higher in FSBM group than that in SBM, FFSBM, and ESBM groups(P<0.05). The findings indicated that FSBM showed the best replacement effect of fish meal, followed by ESBM group, and the worst of FFSBM group in diet of Rhynchocyprislagowskii Dybowski.
2019, Vol. 34 
