Compositional characteristics and in vitro antitumor activities of polysaccharides from alga Isochrysis zhanjiangensis treated with different deproteinizing methods
College of Food Science and Engineering, NationalResearch & Development Branch Center for SeaweedProcessing, Key Laboratory of Aquatic Product Processingand Utilization of Liaoning Province, Dalian OceanUniversity, Dalian 116023, China
Abstract: The crude polysaccharide(F) was preparedfrom microalga Isochrysis zhanjianggensis through alcohol precipitation, and the crude fraction F was processed using Sevag reagent or trichloroacetic acid(TCA), with deproteinized preparations referred as FS and FT, respectively, in order to explore the suitable deproteinization method. The total sugar content, sulfate content and monosaccharide compositions were determined in F, FS and FT, in which the constituents were analyzed by Fourier transform infrared spectroscopy(FT-IR) and gel exclusion chromatography. In addition, inhibitory effects of the three fractions on six tumor cell lines including MCF-7, HeLa, SW480, HT29, HepG2 and A549 were assayed in vitro. It was found that the polysaccharides from the microalga were primarily consisted of mannose, glucose, galactose, arabinose, xylose, glucuronic acid, rhamnose and fucose. The ratios of monosaccharide composition were varied after the polysaccharides were processed using different deproteinizing methods. The high-molecular-weight component(80 000-90 000) was shown to be decreased in F administrated by Sevag, while it was better preserved through TCA treatment. In contrast, the proportion of low-molecular-weight components in FT was reduced due to hydrolysis and dialysis. The antitumor experiment revealed that the maximal function of inhibiting tumor cells was observed in FT, follows by F and the minimal in FS. The findings indicated that polysaccharides treated with different deproteinizing methods showed different compositional characteristics and biological activities, and that the polysaccharide processing technique should be established and optimized based on activity preservation in future commercial exploration.
辛泉伯, 罗薇, 孙可澄, 何云海, 刘舒, 汪秋宽, 宋悦凡. 除蛋白方式对湛江等鞭金藻多糖的组成、结构特征和体外抗肿瘤活性的影响[J]. 大连海洋大学学报, 2019, 34(3): 428-435.
XIN Quan-bo, LUO Wei, SUN Ke-cheng, HE Yun-hai, LIU Shu, WANG Qiu-kuan, SONG Yue-fan. Compositional characteristics and in vitro antitumor activities of polysaccharides from alga Isochrysis zhanjiangensis treated with different deproteinizing methods. Journal of Dalian Ocean University, 2019, 34(3): 428-435.
2019, Vol. 34 
