Effects of crude protein from mucus of the whelk Volutharpa ampullacea Perryi on proliferation of hepatocellular carcinoma and tumor growth of tumor-bearing mice
YU Linghui, ZHAO Tanjun, CHEN Simeng, LI Na, XU Jing, LI Chunmao,ZHAN Yaoyao, CHANG Yaqing*
1.College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China; 2.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs， Dalian Ocean University, Dalian 116023, China; 3.Department of Pathology and Pathophysiology, Dalian Medical University, Dalian 116000, China; 4.Dalian Ocean Island Fisheries Group Company Limited, Dalian 116500, China
Abstract: To preliminarily clarify the anti-tumor effects of crude protein from the mucus of the whelk Volutharpaampullacea Perryi, the effects of crude protein from the mucus of the whelk on the proliferation of human hepatoma Hep-G2 cell and tumor growth of H22 tumor-bearing mice were analyzed and compared in vitro and in vivo experiments using microscopic observation, flow cytometry and qRT-PCR techniques. The results showed that the morphology of Hep-G2 cells in the group treated with 0.66 g/L crude protein from the mucus of the whelk showed severe deformation, nuclear condensation and blurring of nuclear membrane, vacuolation of cytoplasm, swelling of mitochondria and significant reduction of microvilli on the cell surface compared with the untreated control group (as control). The proportion of G2/M stage cells in Hep-G2 cells in the group treated with 0.66 g/L crude protein from the mucus of the whelk was significantly higher than that in the untreated group (P<0.05). The transcript levels of cell cycle protein CCNB1 and cell cycle protein-dependent kinase CDK1 in the Hep-G2 cells were shown to be significantly lower in the crude protein-treated group than those in the control group (P<0.05). The mean tumor weights of the tumor-bearing mice were significantly lower in the crude protein-injected groups (the injection dose of prevention group and the treatment group was 30 mg/kg) than those in the model control group (P<0.01). The findings indicate that crude protein from the mucus of the whelk has anti-hepatocellular carcinoma effects both in vitro and in vivo, showing the potential to be developed as an anti-hepatocellular carcinoma drug of marine origin.
于凌慧, 赵谭军, 陈思梦, 李娜, 徐静, 李春茂, 湛垚垚, 常亚青. 皮氏蛾螺黏液粗蛋白对肝癌细胞增殖及荷瘤小鼠肿瘤生长的影响[J]. 大连海洋大学学报, 2022, 37(4): 592-601.
YU Linghui, ZHAO Tanjun, CHEN Simeng, LI Na, XU Jing, LI Chunmao, ZHAN Yaoyao, CHANG Yaqing. Effects of crude protein from mucus of the whelk Volutharpa ampullacea Perryi on proliferation of hepatocellular carcinoma and tumor growth of tumor-bearing mice. Journal of Dalian Ocean University, 2022, 37(4): 592-601.
2022, Vol. 37 
