Abstract: The aim of this study is to investigate the effects of different acclimation temperature on mitochondrial metabolism in liver of juvenile Amur sturgeonAcipenser schrenckiiBrandtwith body weight of(6.26±0.21)g.The mitochondrial protein contents,mitochondrial respiration rates and mitochondrial cytochrome C oxidase activity (CCO)weremeasured in liver of the fish acclimated at constant temperature of16,19,22 and 25℃and four variable temperature(16±2),(19±2),(22±2)and(25±2)℃for 6 weeks.The results showed that there were no significant differences in mitochondrial protein contents in the fish between acclimation groups(P＞0.05).However,themitochondrial state 3 and state 4 respiration rateswere found to be increased significantly with increases in acclimation temperature(P＜0.05),without significant differences in acclimation groups of 16℃and 19℃(P＞0.05),even though therewere significanthighermitochondrial respiration rates in variable temperature groups than those in constant temperature groups in acclimation groups of22℃and 25℃(P＜0.05).The RCR values ofmitochondrial respiration in liver were declined significantly with increases in acclimation temperature(P＜0.05), without significant differences between constant temperature groups and variable temperature groups in all acclimation temperature(P＞0.05).With increases in acclimation temperature,themitochondrial CCO activitieswere increased significantly(P＜0.05),without significant differences between constant temperature groups and variable temperature groups(P＞0.05).The findings indicate that variable temperature acclimation from 20℃to 27℃can lead to significantly induce increases in mitochondrial respiration rates in liver of juvenile Amur sturgeon.
赵志刚, 李晋南, 徐奇友, 罗亮, 王常安, 王连生, 都雪, 王海. 不同驯化温度对施氏鲟幼鱼肝脏线粒体代谢的影响[J]. 大连海洋大学学报, 2016, 31(6): 607-611.
ZHAO Zhi-gang, LIJin-nan, XU Qi-you, LUO Liang, WANG Chang-an, WANG Lian-sheng, DU Xue, WANG Hai. Effects of different acclimation tem perature on m itochondrial metabolism in liver of juvenile Amur sturgeonAcipenser schrenckiiBrandt. Journal of Dalian Ocean University, 2016, 31(6): 607-611.
2016, Vol. 31 
