1.Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian Province,Xiamen 361013,China;2.Laboratory of Molecular Ecology and Application of Marine Microorganisms,Zhejiang Ocean University,Zhoushan 316022,China
Abstract: In this study,the complete mitogenome sequences of 24 species in Atyidae whose members are small in size,and difficult to make the morphological identification and classification were analyzed by using DnaSP 6,PhyML 3.1,MEGA 5.0,MrBayes 3.2.6 and BEAST 2.6.3 software.The results showed that the lengths of 24 mitogenomes were shown to be changed from 15 318 bp to 16 430 bp,with AT content varying from 62.20% to 70.19% and consistent gene composition and arrangement of mitogenome in 24 species.The analysis of differing sites of mitogenome revealed that the ratio of variable sitesnd5 andnd4 genes were relatively high,with the maximal number of variable sites.The phylogenetic trees based on 13 PCGs (protein-coding genes) showed that the subfamily of Paratyinae was a monophyletic,and that Typhlatyinae and Caridellinae were non-monophyletic which were closely related to each other and clustered into a large branch,forming a sister group with Atyinae alone.Molecular clock analysis suggested that the most recent common ancestor of species in Paratyinae,Typhlatyinae and Caridellinae might occur from the middle Carboniferous to Permian time,while the most recent common ancestor of species in Atyinae might occur from the early Permian Jurassic time,and then further differentiated into the modern characteristic species in Atyidae.In conclusion,nd5 andnd4 genes can be used as ideal molecular markers for species genetic analysis in species in Atyidae.The monophyleticity of Typhlatyinae and Caridellinae is still controversial,and the movement of geographic plates has an important impact on the species differentiation in Atyidae.
2022, Vol. 37 
