Abstract: In order to explore the mechanism of changes in quality of scallop during quality determination period(QDP), pH, glycogen and metabolomics of the adductor muscles were analyzed in post-harvest live Yesso scallop Patinopectenyessoensis with shell length of(11.01±0.31)cm held at 6.9 ℃(control group), and storage in the water(group C)for 36 h(QDP)at 0, 3, 6, 12, 24 and 36 h by an ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS)to analyze the metabolic characteristics of post-harvest Yesso scallop in seawater storage during QDP.The results showed that pH and glycogen contents were found to be slight fluctuation in the Yesso scallop in group C.By multivariate statistical analysis revealed that 7 discrimination metabolites were detected in the Yesso scallop in group C for 36 h, including behenic acid, tyramine, prolyl-glutamate, ADP-glucose, UDP, 3-phosphoserine and citrate.The pathway analysis showed that the most easily affected metabolic pathway was TCA cycle.The findings indicated that live Yesso scallops wet stored for 36 h did not change much in physiological characteristics of initial status during quality determination period, compared with the traditional physical and chemical indicators, with significant differences in metabolome, indicating that the metabolomics could be more sensitive and comprehensively reflecting the real-time state of live scallops during wet storage.
徐昙烨, 田元勇, 李亚烜, 刘俊荣. 基于UPLC-MS方法的品质易逝期活品虾夷扇贝代谢组学研究[J]. 大连海洋大学学报, 2021, 36(4): 637-645.
XU Tanye, TIAN Yuanyong, LI Yaxuan, LIU Junrong. Metabolomics of live Yesso scallop Patinopecten yessoensis during quality determination period by UPLC-MS method. Journal of Dalian Ocean University, 2021, 36(4): 637-645.
2021, Vol. 36 
