Cloning and expression of H-FABP gene in tissues of culter Culter alburnus in Xingkai Lake
HAN Ying1,2, CHEN Xiao-ting1,2, WANG Kun3, ZHANG Hong1,2
1.Animal Science and Technology College, Northeast Agricultural University, Harbin 150030, China;2. Country Location United Engineering Laboratory of Freshwater Fish Breeding, Harbin 150030, China;3. Harbin University of Commerce, Harbin 150028, China
Abstract: The 778 bp cDNA sequence including whole CDS sequence (402 bp), whole 3′UTR sequence (242 bp) and partial 5′UTR sequence (134 bp) of heart type fatty acid binding protein (H-FABP) in culter Culter alburnus was cloned by RT-PCR and RACE technology to evaluate difference between the amount of expression of H-FABP gene in wild and farmed culter in Xingkai Lake, according to the H-FABP gene sequences of members in Cyprinidae known in Genbank with accession number of KC782835. The Blast showed that there was 91% homology in H-FABP gene CDS area between culter and common carp Cyprinus carpio and prenant’s schizothoracin Schizothorax prenanti. The comparison of amino acid homology revealed that the H-FABP gene of the culter showed 76% homology with rainbow trout Oncorhynchus mykiss and 85% homology with zebrafish Danio rerio.The expression of H-FABP gene in 7 tissues (gill, heart, intestine, hepatopancreas, muscle, kidney, spleen) of wild Culter alburnus and in 6 tissues except heart of cultured culter indicated that the maximal mRNA expression of H-FABP gene was observed in hepatopancreas of wild and cultured culter, with significantly different from other tissues (P<0.05). In the same tissue, however, there was no significant difference in mRNA expression level of H-FABP gene in wild and cultured culter (P>0.05). It is concluded that the conservative H-FABP gene in culter had high homology with that in members in Cyprinidae.
2015, Vol. 30 
