Histological observation of gonadal development of topmouth culter Culter alburnus in Jinjiang River
LI Zhong-li;BA Jia-wen;WEI Wen-yan;JIANG Tian-yong;CAI Hong-yan
College of Agroforestry Engineering and Planning, Tongren University, Tongren 554300, China;Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China
Abstract: Morphological and histological development of gonad and germ cells were annually observed in topmouth culter Culter alburnus with body weight of(22.2-2587.5)g by paraffin section technique and H.E staining method. The topmouth culter had testis made up of lobules with spermatogenic cells in cysts, and the development of testis was divided into 6 stages. Spermatogenesis experienced 5 phases: spermatogonia, primary spermatocytes, secondary spermatocytes, spermatid, and spermatozoa, and the developments of ovary were divided into 6 stages, and oogenesis experienced 6 phases. The nucleoli were observed at phase Ⅰ, and increased in number at following phase Ⅱ and Ⅲ. The follicle membrane and yolk nuclei were found at phase Ⅱ, yolk nuclei disappeared at phase Ⅲ, and yolk granules appeared at the late phase Ⅲ, at the same phase follicle membrane differentiated into two layers, the vacuole was formed and changed from one layer to a multilayer, with a light red flocculent area in each of it. The zona radiata were presented at the surface of egg membrane from phase Ⅲ to Ⅴ, yolk granules accumulated at phase Ⅳ, vacuoles were squeezed at the outer edge of cytoplasm, and the nuclei deviated to the edge at late phase Ⅳ. The oocytes were filled with yolk granules at phase Ⅴ, being fused into pieces, and nuclei disappeared, and cortical alveolus became evident. The unspawned ova were broken down and absorbed at phase Ⅵ, and lifted a lot of postovulatory follicles in the oocyte. There existed degenerated cells in the oocyte from phase Ⅱ to Ⅵ, with asynchronous developmental oocytes and spermatocytes. The findings might be used to provide basic data for resource conservation and artificial breeding of topmouth culter in Jinjiang River.
2019, Vol. 34 
