Abstract: The morphology and histological changes were observed in the regenerated body walls in sea cucumber Apostichopus japonicus whose epidermis and the connective tissue layer were cut off and reared in the sea water by naked eye and a light microscopy.The CK19 was expressed in the mice by fluorescence radioimmunoassay.A thin milky connective tissue was found in the body cavity membranes and hollowness was visible 1 day after regeneration.Pigment was appeared 7 days after regeneration,and no hollowness 11 days after regeneration.21days after regeneration,the wound epidermis configuration and color was recovery to the original.Histologically,1 day after connective tissue was exposed directly,and in fibroblasts and collagen congregated fibers were the vicinity of trauma surface.3 days after regeneration,a large number of epithelial cells were in the outer layer of connective tissue, and 4 days after regeneration,a band gap became obvious between the connective tissues.7 days after regeneration, the keratose layer appeared out of the epidermis layer,and 11 days after regeneration,the thickness of epidermis layer and keratose layer were consistent with the normal state.21 days after regeneration,the number and arrangement of epidermis cells were the same as the normal tissue,and connective tissue was homogeneous.The confocal microscopy analysis showed that the natural body wall of the sea cucumber had expression of CK19 dispersedly.3 days after regeneration,the CK19 was expressed in the vicinity of trauma surface.
2011, Vol. 26 
