Chemistry and Biology Engineering College,Yancheng Institute of Technology,Key Laboratory of Aquaculture and Ecology of Coastal Pond of Jiangsu Province,Yancheng 224051,China
Abstract: The alkaline phosphatase(AKP)and acid phosphatase(ACP)in muscle and hepatopancreas,glutamic -oxalacetic transaminase(GOT)and glutamic-pyruvic transaminase(GPT)in hepatopancreas were determined in red swamp crayfish Procambarus clarkia with body weight of(11.35±1.52)g exposed to different concentrations of Cu(Ⅱ)(0.055,0.28,1.38,6.88,and 34.38 mg/L)or Cd(Ⅱ)(0.0048,0.024,0.12,0.60,and 3.00 mg/L)at water temperature of(19±1)℃for 96 h.The results showed that low dose Cu(Ⅱ)and Cd(Ⅱ)had induction of activity of metabolic enzymes in the red swamp crayfish.Cu(Ⅱ)and Cd(Ⅱ)were found to result in increase in metabolic enzyme activities first and then decline.The maximal ACP,AKP,GPT and GOT activities in the hepatopancreas were observed at 0.12 mg/L Cd(Ⅱ),increased by 58.83%,86.17%,85.66%,70.98% compared to the control group(P＜0.05).The maximal ACP and AKP activities were found in the hepatopancreas of the crayfish exposed to 0.28 mg/L Cu(Ⅱ),increased by 112.53%,and 94.98%compared to the control group (P＜0.05),respectively.In the muscle,however,the maximal ACP and AKP activities were found at 1.38 mg/L Cu(Ⅱ),significantly increased by 242.35%and 171.97%compared to the control(P＜0.05).
2011, Vol. 26 
