Abstract: The activities of immune-related enzymes, content of malonaldehyde (MDA) in coelomic fluid and accumulation of Cu(Ⅱ)in shell were determined in sea urchin Strongylocentrotus intermedius exposed to the various Cu(Ⅱ) concentrations designed by marine water quality standard to focus on physiological effects of Cu(Ⅱ) on echinoderms. The results showed that the mortality in the sea urchin was significantly increased with the elevated concentration of Cu(Ⅱ), with 24 h LC50 of 0.247 mg/L. The superoxide dismutase (SOD) was induced in the sea urchin in 0.012 mg/L group, but inhibited in other groups in which the SOD activity was increased firstly and then decreased. In all treatment groups, the activity of catalase(CAT) was induced once but inhibited, the minimum on 30 d and, significantly lower than that in the control group (P<0.05). The activity of peroxidase(POD) was found to be increased with the rise of the concentrations of Cu(Ⅱ), the maximum on 30 d, and significantly higher than that in the control group (P<0.05). In 0.012 mg/L group, lysozyme (LZM) was induced first but inhibited, and the activity of LZM was induced in other treatment groups. In all treatment groups, the content of MDA was induced once but inhibited. The accumulation of copper ions was found to be increased with copper ion exposure elapse. The findings provided a new sight on the physiological effects of Cu(Ⅱ)and on monitoring of Cu(Ⅱ)levels in marine with bio-detection.
2015, Vol. 30 
