Regulation of L-carnitine on antioxidant function in Pimephales promelas muscle cell line and grass carp Ctenopharyngodon idellus ovary cell line
ZHENG Nan1, WANG Qiu-ju1, MA Yue1, YU Ting1, CHEN Yu-ke1, LIU Hong-jian2, ZHANG Dong-ming3，1
1.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2.Fishery Technical Extension Station of Jilin Province, Changchun 130012, China; 3.Tonghua Normal University, Tonghua 134000, China
Abstract: The activities of Copper, Zinc-superoxide dismutase (CuZn-SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione ligase synthesis subunit (GCLC) and the gene relative expression of m RNA’s of CuZn-SOD, CAT, GPX and GCLC were investigated in fathead minnowPimephalespromelas muscle cell line (FHM) for 6 h and grass carpCtenopharyngodonidellus ovary cell line (GCO) for 12 h in culture media containing L-carnitine at a dose of 0, 0.001, 0.01, 0.1, 0.5, 1 and 5 mmol/L by q-PCR to evaluate the regulatory function of L-carnitine on the gene relative expression of antioxidant enzymes in FHM and GCO. The results showed that no significant differences in CuZn-SOD mRNA relative expression was found in the FHM between the L-carnitine groups and the control group(P>0.05). There was significant higher regulated CuZn-SOD mRNA relative expression in 0.5 and 5 mmol/L L-carnitine group than that in the control group in the GCO (P<0.05). Compared to the control group, there was significantly higher activities of SOD and CAT in 0.01-5 mmol/L L-carnitine group in the FHM (P<0.05). In the 5 mmol/L group, however, there were significantly higher activities of SOD, CAT, GPX and γ-GCS in GCO cells than those in the control group (P<0.05). The significant up-regulation in CAT mRNA relative expression were found in GCO between all of the L-carnitine groups and the control groups (P<0.05) and in FHM cells in 0.01-5 mmol/L carnitine groups (P<0.05). The GPX mRNA relative expression in GCO cells was shown to be significantly up-regulation in 0.5 mmol/L L-carnitine group (P<0.05), with significantly up-regulated GCLC mRNA relative expression in 0.1 mmol/L L-carnitine group compared to the control group in GCO (P<0.05). The level of GCLC mRNA relative expression was significantly increased when the GCO was treated by 0.5, 1 and 5 mmol/L L-carnitine (P<0.05). The findings indicate that supplementation of L-carnitine up-regulates the mRNA relative expression of antioxidant enzymes in FHM and GCO, and it is suggested that the appropriate concentration of L-carnitine be 0.1-1 mmol/L under the conditions in present experiment.
2018, Vol. 33 
