虾青素对褶皱臂尾轮虫种群增长的影响及其富集效果

doi:10.16535/j.cnki.dlhyxb.2021-302

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大连海洋大学学报 ›› 2022, Vol. 37 ›› Issue (2) : 268-275. DOI: 10.16535/j.cnki.dlhyxb.2021-302

虾青素对褶皱臂尾轮虫种群增长的影响及其富集效果

刘辉,孙娜,姜玉声,许祥,王澳林,张乃达,张冰倩

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Effects of dietary astaxanthin on population growth of rotifer Brachionus plicatilis and astaxanthin accumulation efficiency

LIU Hui, SUN Na, JIANG Yusheng, XU Xiang, WANG Aolin, ZHANG Naida, ZHANG Bingqian

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摘要

为了探究虾青素对褶皱臂尾轮虫Brachionus plicatilis种群增长的影响及其富集效果，在水温为23～25 ℃、海水盐度为18的条件下，以仅投喂微拟球藻Nannochlorsis sp.为对照组，并在此基础上以分别添加0.05、0.10、0.15 mg/L虾青素作为处理组培养轮虫，初始接种密度为10 ind./mL，连续培养7 d，同时进行12 h虾青素富集培养试验。结果表明：0.15 mg/L虾青素添加组，轮虫最高密度、日均增长量、日均增长率和最终增长倍数均最高，分别为134 ind./mL、17.1 ind./(mL·d)、36.6%/d和13.0倍，显著高于0.05 mg/L虾青素组和对照组(P<0.05);0.10、0.15 mg/L虾青素添加组培养至4 h时，轮虫体内的虾青素含量均达到最大值(分别为17.02、19.46 μg/mg)，其富集效率也较高(分别为3.99、4.60 μg/(g·h))，两组的这些指标均显著高于其他组(P<0.05)，随着培养时间的延长，3个处理组轮虫富集虾青素的效率逐渐降低，并趋于平稳；0.15 mg/L虾青素组培养水体中氨氮质量浓度最高(2.40 mg/L)且显著高于0.05 mg/L虾青素组和对照组(P<0.05)；各组轮虫培养水体中亚硝酸盐、pH变化比较平稳且无显著性差异(P>0.05)；各组水体溶解氧含量与轮虫密度呈负相关，0.15 mg/L虾青素组溶解氧含量显著低于其他组(P<0.05)。研究表明，本试验条件下，在微拟球藻藻液中添加虾青素投喂褶皱臂尾轮虫，有利于轮虫种群增长且可有效富集虾青素；从富集效率和成本等方面综合考虑，以0.10 mg/L的虾青素添加量对轮虫进行强化培养4 h,投喂水产苗种较为合适。

Abstract

In order to explore effects of astaxanthin on population growth of rotifer Brachionus plicatilis and enrichment efficiency, the rotifer was cultured in a 120 L plastic tank at initial inoculation density of 10 ind./mL and fed microalga Nannochlorsis sp.(control group), and the microalga supplemented with astaxanthin at a dose of 0.05, 0.10 and 0.15 mg/L as the treatment groups at water temperature of 23-25 ℃ and salinity of 18 for 7 d.Meanwhile, 12 h astaxanthin enrichment culture experiment was carried out under the same conditions.Results showed that the maximal rotifer density of 134 ind./mL, average daily growth of 17.1 ind./(mL·d), average daily growth rate of 36.6%/d and final growth multiple fold of 13.0 were observed in the rotifer in 0.15 mg/L astaxanthin addition group, significantly higher than those in 0.05 mg/L astaxanthin group and control group(P<0.05).The rotifer had the maximal astaxanthin contents at 4 h in the rotifer in 0.10 mg/L astaxanthin addition group(17.02 μg/mg)and 0.15 mg/L astaxanthin addition group(19.46 μg/mg), and had high enrichment efficiency(3.99 and 4.60 μg/(g·h), respectively), significantly higher than those in the other groups(P<0.05).The enrichment efficiency of astaxanthin in rotifers in the three astaxanthin addition groups was decreased gradually and stabilized when culture time was prolonged.The maximal concentration of ammonia nitrogen in the culture water was found in 0.15 mg/L astaxanthin group(2.40 mg/L), significantly higher than that in 0.05 mg/L astaxanthin group and control group(P<0.05).The changes in nitrite concentration and pH in the culture water were relatively stable in rotifers in each group, without significant difference(P>0.05).The dissolved oxygen content in each group was negatively correlated with rotifer density, significantly lower dissolved oxygen content in 0.15 mg/L astaxanthin group than that in other groups(P<0.05).The findings indicated that supplementation with astaxanthin into Nannochlorsis sp.to feed rotifer Brachionus plicatilis is beneficial to the growth of rotifer population and can effectively enrich astaxanthin under the experimental conditions, 0.10 mg/L astaxanthin being of suitable dose for intensive culture of rotifer for 4 h, and feeding aquatic seedlings based on the comprehensive analysis of enrichment efficiency and cost.

导出引用

刘辉, 孙娜, 姜玉声, 许祥, 王澳林, 张乃达, 张冰倩. 虾青素对褶皱臂尾轮虫种群增长的影响及其富集效果[J]. 大连海洋大学学报, 2022, 37(2): 268-275 https://doi.org/10.16535/j.cnki.dlhyxb.2021-302

LIU Hui, SUN Na, JIANG Yusheng, XU Xiang, WANG Aolin, ZHANG Naida, ZHANG Bingqian. Effects of dietary astaxanthin on population growth of rotifer Brachionus plicatilis and astaxanthin accumulation efficiency[J]. Journal of Dalian Fisheries University, 2022, 37(2): 268-275 https://doi.org/10.16535/j.cnki.dlhyxb.2021-302

中图分类号： S 963.21