Effects of cadmium( Ⅱ ) stress on xanthine oxidase and antionxidant enzyme activities in hepatopancreas of oriental weatherfish Misgurnus anguillicaudatus
SUN Shu-hong, JIAO Chuan-zhen, LIU Xiao-lin, WEI Zhen-lin
1. College of Animal Science and Technology, Northwest Agricultural & Forestry University, Yangling 712100, China; 2. Department of Biology, Dezhou University, Dezhou 253023, China
Abstract: The activities of superoxide dismutase ( SOD), catalase ( CAT), peroxidase (POD) and malonyldialdehyde(MDA) were determined in hepatopancreas in oriental weatherfish Misgurnus anguillicaudatus exposed to different concentrations [0.025 mg Cd( Ⅱ )/L,0.25 mg Cd(Ⅱ )/L and 2.5 mg Cd(Ⅱ)/L] of Cadmium chloride ( Cd( Ⅱ ) ) to evaluate the effects of cadmium( Ⅱ ) stress on xanthine oxidase and antionxidant enzyme activities. The results showed that there were significant increase in SOD activity in the fish exposed to 0.025 mg Cd( Ⅱ )/L, with the procedure, but no significant changes in activities of CAT, and POD and MDA content. In the high concentration groups (0.25 and 2.5 mg Cd( Ⅱ)/L), however, the antioxidant enzyme activities increased at first and then decreased, the higher the Cd( Ⅱ ) concentration, more rapidly and earlier the antioxidant enzyme activities increased. SOD activity was found to be sensitive to Cd( Ⅱ ) concentration and time changes, followed by CAT and POD activities, and opposite to SOD activity, and MDA content.