1.Key Laboratory ofMarine Bio-resources Restoration and HabitatReparation in Liaoning Province,Dalian Ocean University,Dalian 116023,China; 2.Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China
Abstract: LoachMisgurnus anguillicaudatusfin cell line(DIMF)was used to assess toxicity of potassium dichromate in vitro to study toxicitymechanism of potassium dichromate and establish suitable for chrome pollution detection system.The results showed that themedian lethal concentration(LC50)was(25.3±1.2)μmol/L in DIMF exposed to potassium dichromate for 24 h measured by thiazole blue(MTT)method.The activities of superoxide dismutase(SOD)in DIMF were increased with the increase in concentration within potassium dichromate concentration of0-30μmol/L and the activities of glutathione peroxidase(GSH-Px)were increased at0-20μmol/L potassium dichromate,and then decreased at concentration of 30μmol/L,with gradual decrease in glutathione S-transferase(GST)activity.Micronucleus test revealed that the rate ofmicronucleuswas increased first and then decreased with the increase in potassium dichromate concentration,with themaximum micronucleus rate of0.733％. Real-time PCR showed thatmetallothionein(MT)gene expression quantity was very low in control group,and increased significantly due to the induction by potassium dichromate(P＜0.01).The findings indicate that potassium bichromate can damage enzyme system and genetic materials in cells.
2016, Vol. 31 
