Effects of different application methods of clove oil on anesthetic effect of red swamp crayfish Procambarus clarkii
JIANG Jingjing, ZHANG Chengfeng, HAN Caian, TIAN Can, ZHANG Ping, XU Zenghong, GAO Yang, SU Shengyan*,TANG Yongkai*#br#
1.College of Wuxi Fisheries, Nanjing Agricultural University, Wuxi 214081, China; 2.Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 3.Jiangxi Vocational College of Biotechnology, Nanchang 330220, China; 4.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 5.Suining Agricultural and Rural Bureau, Xuzhou 221200, China
Abstract: In order to reduce the stress damage of red swamp crayfish Procambarusclarkii caused by human operation during the actual production and scientific research, small red swamp crayfish with body weight of (6.17±0.20)g were bathed in and sprayed with the mixture of clove oil and ethanol solution at a volume ratio of 1∶1.5 at a dose of 1, 2, 4, and 8 g/L and 8, 10, 16, and 20 g/L, respectively. Large red swamp crayfish with body weight of (29.54±0.30)g were bathed in and sprayed with the same mixture of clove oil and ethanol solution at a dose of 10, 16, 20, and 24 g/L and 8, 10, 16, and 20 g/L, respectively for anesthesia. The results showed that with the increase in anesthetic agent concentration, the anesthetic time was found to be shortened and the recovery time to be prolonged, with the appropriate concentrations of 8 g/L in small size crayfish and 20 g/L in large size ones, and the appropriate concentrations of 4 g/L for spray anesthesia and 16 g/L for bath. The red swamp crayfish with spraying anesthesia showed cheliped behavior that was used as a criterion to divide anesthesia into four stages. Significant decrease in oxygen consumption and anesthetic effect was observed in both methods of anesthesia (P<0.05). There were significantly lower oxygen consumption rate and SOD and CAT enzyme activities in the red swamp crayfish with spraying anesthesia than those in the crayfish with immersion anesthesia (P<0.05). The findings indicated that both spraying and immersion anesthesia were used for red swamp crayfish anesthesia, especially surface spraying anesthesia being featured by significant decrease in the oxygen consumption rate and less stress damage to individual gill tissue, and better anesthesia effect compared with the immersion anesthesia.
2022, Vol. 37 
