Effects of MS-222 and clove oil on oxygen consumption rate, ammonia excretion rate and asphyxial point of red swamp crayfish Procambarus clarkii
XU Bin， WEI Kai-jin， ZHU Xiang-yun， XU Jin， MA Bao-shan
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
Abstract: Effects of MS-222 (0, 20, 40, 60, 80, and 100 mg/L)and clove oil(0, 8, 16, 24, 32, and 40 mg/L) on oxygen consumption rate, ammonia excretion rate and asphyxial point were investigated in red swamp crayfish Procambarusclarkii with body weight of (4.18± 0.54)g at water temperature of (18.0±0.5)℃ to improve the use of anesthetics in culture and breeding of red swamp crayfish. The oxygen consumption rate and ammonia excretion rate were found to firstly go up， and then to be down as with the anesthetic concentration was increased. Compared with the control group, no decrease in oxygen consumption rate was observed in the crayfish exposed to low concentration (20-100 mg/L) of MS-222, even though decrease in ammonia excretion rate in the crayfish exposed to 100 mg/L MS-222, without significant difference (P>0.05). There was significantly lower oxygen consumption and ammonia nitrogen in the crayfish exposed to 40 mg/L clove oil (P<0.05), and the crayfish had significantly higher asphyxiation points than the crayfish in the control group did (P<0.05). In conclusion, there was no significant anesthetic effect of MS-222 on the crayfish, while it was recommended that clove oil be used for live transportation of red swamp crayfish at concentration of below 40 mg/L within 4 h.
2018, Vol. 33 
