CO2麻醉处理对罗氏沼虾保活的影响

doi:10.16535/j.cnki.dlhyxb.2019-329

PDF(1015 KB)

大连海洋大学学报 ›› 2020, Vol. 35 ›› Issue (6) : 874-882. DOI: 10.16535/j.cnki.dlhyxb.2019-329

CO2麻醉处理对罗氏沼虾保活的影响

杨丰1，王逸鑫1，沈思远1，吴涵1，施文正1、2，汪之和1、2、3*

作者信息 +

Effects of carbon dioxide anaesthesia on survival of giant freshwater prawn Macrobrachium rosenbergii

YANG Feng1, WANG Yixin1, SHEN Siyuan1, WU Han1, SHI Wenzheng1,2, WANG Zhihe1,2,3*

Author information +

文章历史 +

摘要

为提高罗氏沼虾Macrobrachium rosenbergii在保活过程中的存活时间和存活率，探究不同质量浓度CO2(60、120、180、240、300 mg/L，对照组不添加CO2)、不同虾水质量比(1∶3、1∶6、1∶9、1∶12)和不同温度(12、16、20、25 ℃)的麻醉液处理对罗氏沼虾(体质量20～30 g)保活过程的影响，通过正交试验确定CO2麻醉质量浓度及罗氏沼虾保活的最佳条件，检测并分析了最佳麻醉和保活条件下水质指标及罗氏沼虾肌肉、血液、肝脏等组织生理生化指标的变化。结果表明：罗氏沼虾经150 mg/L的CO2麻醉液处理后，在保活温度为18 ℃、虾水质量比为1∶10条件下，48 h的存活率达90%以上，在此条件下水体总氨氮、溶氧量、pH均在罗氏沼虾适宜存活范围内变化，而虾体肌肉三磷酸腺苷(ATP)、肌糖原和肝糖原均不同程度地显著下降(P<0.05)，并产生大量乳酸，引起肌肉pH下降，虾血液谷草转氨酶(AST)和谷丙转氨酶(ALT)活力显著上升(P<0.05)，肝脏总超氧化物歧化酶(T-SOD)活力与丙二醛(MDA)含量呈负相关并显著变化。研究表明，CO2麻醉可有效缓解虾体应激，并能控制水质指标的变化，从而在一定程度上降低虾体肌肉、血液及肝脏各组织代谢，能延长其保活时间并提高存活率。

Abstract

Effects of CO2 anesthesia concentrations of 60, 120, 180, 240, and 300 mg/L on survival time and survival rate of giant freshwater prawn Macrobrachium rosenbergii with body weight of 20-30 g were investigated at prawn to water ratio in weight of 1∶3, 1∶6, 1∶9, and 1∶12 and at water temperature of 12, 16, 20, and 25 ℃. The optimization of CO2 anesthesia and the changes in water quality and physical and chemical indices were evaluated in muscle, blood, liver and other tissues of giant freshwater prawn under the conditions of CO2 concentrations of 150, 180, and 210 mg/L, at water temperature of 18, 20, and 22 ℃ and at prawn to water ratio in weight of 1∶8, 1∶9, and 1∶10 during the keep-alive process in an orthogonal test. The results showed that over 90% of survival within 48 h was observed in the giant freshwater prawn exposed to CO2 anaesthesia concentration of 150 mg/L, at 18 ℃ and at prawn to water ratio in weight of 1∶10. Under these conditions, the levels of total ammonia nitrogen, and dissolved oxygen and pH in water were within the suitable survival range, and levels of adenosine triphosphate(ATP), muscle glycogen and liver glycogen were significantly decreased to some extent(P<0.05). The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in blood were found to be increased significantly. The activity of T-SOD and MDA content in liver were negatively correlated with CO2 concentration and significantly changed. The findings indicated that CO2 anesthesia alleviated the stress of prawn body and controlled the change in water quality index, so as to reduce the metabolism of body muscles, blood and liver tissues to improve the survival time and survival rate to some extent.

导出引用

杨丰, 王逸鑫, 沈思远, 吴涵, 施文正, 汪之和. CO2麻醉处理对罗氏沼虾保活的影响[J]. 大连海洋大学学报, 2020, 35(6): 874-882 https://doi.org/10.16535/j.cnki.dlhyxb.2019-329

YANG Feng, WANG Yixin, SHEN Siyuan, WU Han, SHI Wenzheng, WANG Zhihe, ?. Effects of carbon dioxide anaesthesia on survival of giant freshwater prawn Macrobrachium rosenbergii[J]. Journal of Dalian Fisheries University, 2020, 35(6): 874-882 https://doi.org/10.16535/j.cnki.dlhyxb.2019-329

中图分类号： S981.21