Abstract: The genetic diversity was firstly investigated in 71 samples of Japanese eel Anguillajaponica collected in Jingjiang section of the Yangtze River by AFLP, and then nine pairs of selective amplification primers were chosen and used to analyze the polymorphic site, parameters of genetic diversity, parameters of gene differentiation and the dendrogram through Popgene and Ntsys software, with good separation in nine groups based on captured time and two groups on body length, respectively. Results showed that a total of 216 polymorphic sites were detected through the nine pairs of selective amplification primer, with an average polymorphic site of 24 each pair primer, the maximum percent polymorphic site of 98.15%, the minimum one of 91.20%, and mean percent polymorphic site of 95.06% in the nine groups from E-ACC/M-CAA. Correspondingly, from E-ACC/M-CTG, the maximum was observed 100% in July, August and September, while, the minimum 93.06% in March and May. Simultaneously, there was the mean Nei value of 0.277 2 and Shannon index of 0.433 2 in the nine groups, the maximum in July, and the minimum in September and November. There were higher effective number of alleles, Nei’s index and Shannon index in group A (body length>400 mm) than those in group B (body length<400 mm). AMOVA revealed that more than 80% of variation was derived from individuals both in nine groups and body length groups (A and B). The findings indicated that the Japanese eel had high level of genetic diversity, which was probably benefited from the primerly geographic position and migratory habit. Concurrently, two prominent branches in dendrogram suggested that not all individuals came from the same parent, and the differentiation level and gene flow was still existed in these samples.
杨彦平, 徐东坡, 方弟安, 刘凯. 基于AFLP标记研究长江靖江段日本鳗鲡群体遗传多样性[J]. 大连海洋大学学报, 2018, 33(4): 460-466.
YANG Yan-ping, XU Dong-po, FANG Di-an, LIU Kai. Analysis of genetic diversity of Japanese eel Anguilla japonica from Jingjiang section of the Yangtze River through AFLP. Journal of Dalian Ocean University, 2018, 33(4): 460-466.
2018, Vol. 33 
