Abstract: The biochemical composition was determined in south flounder Paralichthys lethostigma juveniles weighing (7.56 ± 0.63 ) g refed for 20 days 5 ( S5 ), 10 ( S10 ) and 15 ( S15 ) days after starvation to evaluate the effect of starvation and refeeding on the biochemical composition. The results showed that body weight loss of 1. 18%, 8.36% and 11.76% was observed in S5, S10, S15 during the starvation, the body weight in group S15 being significantly lower than that at the beginning(P 〈 0.05 ). There was no significant difference in protein content in whole body in the S5 and S10, but significantly lower protein content in the S15 than that in the control (P 〈0.05). The lipid content in the whole body gradually decreased, and significantly lower than that in the control ( P 〈 0.05 ) while the water content increased, significantly higher in S15 (P 〈 0.05 ). There was higher ash content in the whole body in the fish starved than that in the control with significant differences in the S10 and S15 ( P 〈 0.05 ). During the refeeding, the protein content gradually increased while water and ash contents gradually decrease in all in S15, and the lipid content in the body gradually increased starvation groups.