Cloning of polymeric immunoglobulin receptor gene fragment in grass carp Ctenopharyngodon idellus using degenerate primers designed by CODEHOP
XU Guo-jing, WANG Chao, MENG Qing-lei, LIU Feng, LIU Fei，CHEN Xiu-li
Saline-alkali Fishery Engineering Technology Research Center of Shandong Province, Freshwater Fisheries Research Institute of Shandong Province, Jinan 250013, China
Abstract: The gene of polymeric immunoglobulin receptor(pIgR)of grass carp Ctenopharyngodon idellus was cloned by a pair of degenerate primers designed by CODEHOP, and by combined bioinformatics including NCBI, Blast and biological software such as Primer Premier 5.0 and DNAMAN, and the homology and phylogenetic tree of the clone of pIgR gene were analyzed in the PCR products. The results showed that degenerate primers designed by CODEHOP were characterized by specificity and benefit for the experiment accomplishing. pIgR gene of grass carp was found to clustered into pIgR gene group in all the other vertebrates, especially into the fish subgroup, with the maximal homology (84%) with common carp Cyprinus carpio.
许国晶, 王超, 孟庆磊, 刘峰, 刘飞, 陈秀丽. 用CODEHOP法设计简并引物克隆草鱼多聚免疫球蛋白受体基因片段[J]. 大连海洋大学学报, 2015, 30(2): 138-142.
XU Guo-jing, WANG Chao, MENG Qing-lei, LIU Feng, LIU Fei, CHEN Xiu-li. Cloning of polymeric immunoglobulin receptor gene fragment in grass carp Ctenopharyngodon idellus using degenerate primers designed by CODEHOP. Journal of Dalian Ocean University, 2015, 30(2): 138-142.
2015, Vol. 30 
