YAO Dong-lin1,2,ZOU Qing1,2,LIU Wen-bin3,XIE Shao-lin1,ZHOU Ai-guo1, CHEN Jin-tao1,ZOU Ji-xing1
1.College of Animal Science,South China Agricultural University, Guangzhou 510642,China;2.Qingyuan North River Fishery Science Institute, Qingyuan 511500, China;3.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095,China
Abstract: A total of 300 grass carpCtenopharyngodonidellajuveniles with initial body weight of (71.1±1.4)g were randomly divided into a 2 m×2 m×1.5 m cage and fed basal diet in the control group and the basal diet supplemented withBacilluslicheniformisand xylo-oligosaccharide in the test group at a rate 0.1% at a feeding rate of about 3.5% at water temperature of 24-33 ℃ for 92 days with triplication. The results showed that there were 20.9% weight gain rate and 8.9% lower food conversion ratio compared with the control group, weight rate in the test group than those in the control group, with significant difference (P<0.05).The number ofBacillussp. in the intestine was found to be decreased by 9.4% in the test group while the number ofBacilluslicheniformisandLactobacillussp. was found to be increased by 21.6% and 7.7%, respectively, with significant differences (P<0.05). There were 18.8% higher trypsin, 36.8% higher amylase, and 19.8% higher lipase activities in the intestines in the grass carp juveniles fed the diets containing synbiotics than those in the juveniles in the control group. In the patopancreas, however,the fish fed the diets containing synbiotics had 14.6% higher trypsin, and 21.3% higher lipase activities than the animals in the control group did(P<0.05), without significant difference in amylase activity.