Optimal fermentation medium and growth in a fermentor of Pseudomonas aeruginosa inactivated vaccine in grass carp
SUN Cheng-wen1, LAI Ying-tiao1, REN Xiao-bo2, JIANG Xiao-yan1, GONG Hua1, HUANG Zhi-bin1, TAO Jia-fa1
1.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Abstract: The nitrogen source, carbon source and phosphate were screened in culture medium of Pseudomonasaeruginosa inactivated vaccine in a single-factor test, and different ingredients of the medium were optimized in an orthogonal experiment, with a verification test of growth of the bacterium in a 5 L fermentor.It was found that the best fermentation of Pseudomonasaeruginosa in grass carp Ctenopharyngodonidellus with red skin disease was observed under the conditions of peptone+beef extract+yeast extract as nitrogen source, glucose as carbon source, K2PHO4 as phosphate source, at a dose of peptone 10 g/L, beef extract 5.0 g/L, yeast extract 2.5 g/L, glucose 5.0 g/L, K2HPO4 0.75 g/L, and NaCl 5.0 g/L.The fermentation kinetics analysis revealed that the stain had the maximal cell density at 6.44 of OD600 nm in 14 h culture, indicating that a high yield of Pseudomonasaeruginosa JP802 fermentation broth is obtained by the optimized fermentation medium.
孙承文, 赖迎迢, 任小波, 江小燕, 巩华, 黄志斌, 陶家发. 草鱼铜绿假单胞菌灭活疫苗发酵培养基的优化及其在发酵罐的生长试验[J]. 大连海洋大学学报, 2019, 34(1): 15-20.
SUN Cheng-wen, LAI Ying-tiao, REN Xiao-bo, JIANG Xiao-yan, GONG Hua, HUANG Zhi-bin, TAO Jia-fa. Optimal fermentation medium and growth in a fermentor of Pseudomonas aeruginosa inactivated vaccine in grass carp. Journal of Dalian Ocean University, 2019, 34(1): 15-20.
2019, Vol. 34 
