Abstract: The cloning and prokaryotic expression of defensin db-1 gene were studied and the recombinant protein of defensin db-1 gene was obtained in redfin puffer Takifugu rubripes by RT-PCR. The mature peptide coding sequences of defensin db-1 gene was inserted into the plasmid pET-32a+ to construct the prokaryotic expression vector pET32a-mDB-1, which was transformed into Escherichia coli competent cells Rosetta(DE3) and was expressed after induced by IPTG. The protein expression was detected by SDS-PAGE gel electrophoresis and Western Blotting, and the expressed fusion proteins were purified by affinity chromatography with Ni-NTA. Finally, the fusion proteins were identified by the Elisa with the probe of goat Anti-Mus Defensin Beta2 antibody. The defensin db-1 gene was found to be expressed effectively in E.coli, and the recombinant protein has been purified and obtained confidently. The findings provided a simple and efficient way to get the recombinant defensin protein, and a support for the production and application of this protein.
2015, Vol. 30 
