LIU Hai-ying1,ZHANG Hong1,CHEN Lei1,XING Kun1,YU Xiao-ming1,TIAN Yi2
1.Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023,China; 2.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
Abstract: The expression of Mn-SOD gene was detected in pseudozoea (Z9-Z11) of mantis shrimpOratosquillaoratoriawhen the pseudozoea was exposed to a salinity of 12.8, 16.3, 19.8, 23.3, 26.8(control group), 30.3, 33.8, and 37.3 for 1.5, 3, 6, 9, 12, 24, 36, 48,and 72 h by real time PCR. The results showed that within the different salinity exposure of 6,9,12,48,and 72 h there was very significantlys and significantly lower Mn-SOD gene expression levels than that in the control group (P<0. 01 orP<0.05) and there was mostly higher Mn-SOD gene expression levels in salinity of 23.3,and 30.3 than that in the 37.3 or 12.8 groups (P<0. 01). In the salinity exposure from 24 h to 36 h, there was significantly higher Mn-SOD gene expression levels than that in the control group except salinity of 12.8, and 16.3 than that in the control group (P<0.01). The gene expressions in different salinity stress were found to be decrease firstly, then increased and finally decreased gradually during 72 hours except the maximal and minimal salinity. At a salinity of 12.8, 16.3, 19.8, 23.3, 30.3, 33.8,and 37.3, the maximum gene expression was observed in 1.5, 12, 24, 24, 24, 24,and 24 h salinity exposure，respectively. The mantis shrimp larvae had a salinity adaptation trend from unadaptation, via the most unadaptation, and gradual adaptation, to unadaptation.
2014, Vol. 29 
