Effects of different cold stress methods on antioxidant enzyme activity and lipid peroxidation of zazor clam Sinonovacula constricta during keeping alive under air exposure
HAO Shuang1, ZHANG Min1,2,3*
1.College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2.Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China; 3.National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China
Abstract: Three groups of zazor clam Sinonovaculaconstricta with average shell length of 59 mm were held in a 400 mm×300 mm×120 mm plastic box covered with absorbent sponge at bottom and exposed to three cold stress methods: acute cooling from (20.0±0.5)℃ to 4 ℃, gradient cooling (from 20.0 ℃±0.5 ℃ to 15.0 ℃±0.5 ℃, 90 min later to (10.0±0.5)℃, and then 90 min later to (4.0±0.5)℃ at a rate of 5 ℃/h) and linear cooling (from 20.0 ℃±0.5 ℃ to 4.0 ℃±0.5 ℃ at a rate of 5 ℃/h), and then stored in a refrigerated storage room at 4 ℃ for keeping alive under air exposure. During the storage, changes in activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and hydrogen peroxide (H2O2)and contents of reduced glutathione (GSH) and malondialdehyde (MDA) were determined in gill and digestive gland of zazor clam exposed to different cold stress methods 0, 2, 4, 6, and 8 d after the storage to evaluate the effects of different cold stress methods on the oxidative stress of zazor clam during keeping alive under air exposure and low temperature and to probe into suitable cold stress methods to reduce oxidative stress damage during storage. The results showed that there was lower SOD activity in the gradient cooling group and the linear cooling group than that in the acute cooling group, significantly lower than that in the acute cooling group on the 6th day (P<0.05). The CAT activity was shown to be lower in the gradient cooling group and the linear cooling group than that in the acute cooling group, significantly lower than that in the acute cooling group on the 6th and 8th day (P<0.05), downward and relieved trend on the 8th day. The POD activity was lower in the gradient cooling group and the linear cooling group than that in the acute cooling group, and decreased to the pre-cooling level on the 4th day of preservation. There was significantly lower GSH content in the gradient cooling group and the linear cooling group than that in the acute cooling group (P<0.05) on the 4th day, and gradually decreased on the 4th, 6th and 8th day, with significant inhibition effect on oxidative stress. The significantly lower MDA content was found in the gradient cooling group and the linear cooling group than that in the acute cooling group (P<0.05) on the 0 day to 8th day when increase rate was slow, with less lipid peroxidation damage in the cell membrane. The H2O2 content was significantly lower in the gradient cooling group and the linear cooling group than that in the acute cooling group on the 2nd, 4th and 8th day of preservation (P<0.05), which prevented from the further increase in oxidative stress and protected the body from poisoning. The findings indicated that the gradient-cooling and linear cooling preservative treatments had little effect on the antioxidant enzyme activity and lipid peroxidation during the preservation period in zazor clam,and alleviated the oxidative stress state of zazor clam under low temperature stress compared with the acute cooling, which is beneficial to the prolongation of period of keeping alive and to the recovery of the antioxidant system of zazor clam.
郝爽, 张敏. 不同冷胁迫方式对缢蛏无水保活期抗氧化酶活性及脂质过氧化的影响[J]. 大连海洋大学学报, 2020, 35(4): 584-590.
HAO Shuang, ZHANG Min, . Effects of different cold stress methods on antioxidant enzyme activity and lipid peroxidation of zazor clam Sinonovacula constricta during keeping alive under air exposure. Journal of Dalian Ocean University, 2020, 35(4): 584-590.
2020, Vol. 35 
