Microwave-assisted preparation and properties of carboxymethyl chitosan(CMC) in Antarctic krill Euphausia superba
CHEN Luzhu, SHI Wenzheng, WANG Zhihe
1.College of Food Sciences and Technology， Shanghai Ocean University, Shanghai 201306, China; 2.National Freshwater Aquatic Products Processing Technology Research and Development Center, Shanghai 201306, China; 3.Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China
Abstract: In order to probe into a rapid and efficient method for preparing carboxymethyl chitosan(CMC), CMC was prepared from Antarctic krill Euphausiasuperba shells by microwave assisted method through single factor test and orthogonal test of four factors, namely, microwave power, microwave time, 40% sodium hydroxide solution dosage, and 50% chloroacetic acid-isopropanol solution dosage, with CMC substitution degree as the evaluation index to determine the best process parameters. The structure of the obtained product was characterized, and the water solubility and antioxidant properties of CMC were analyzed. The results showed that the best preparation effect was observed under the conditions of microwave power of 540 W for 30 min, 40% sodium hydroxide solution of 100 mL, and 50% chloroacetic acid-isopropanol solution of 40 mL, with 0.95 of substitution of carboxymethyl chitosan of Antarctic krill. Characterization analysis showed that the carboxymethyl chitosan of Antarctic krill was N, O carboxymethyl chitosan showing good water solubility at pH of 4-12, and superior to the Antarctic krill chitosan(CS) in hydroxyl scavenging ability and total antioxidant capacity. The findings indicated that the microwave-assisted method significantly improved the preparation efficiency of Antarctic krill CMC, and that the obtained CMC had good water solubility and antioxidant properties, which provide theoretical basis for the application of CMC as a water-soluble antioxidant.
2021, Vol. 36 
