Abstract: A 56 day trial with triplication was conducted to evaluate the effects of five isoenergetic diets containing protein levels of 25%(CP25 group),30%(CP30 group),35%(CP35 group),40%(CP40 group)and 45% (CP45 group)on growth performance and blood parameters in Pacific white leg shrimp Litopenaeus vannamei with body weight of 0.35 g reared in a 300 L plastic farming system at a rate of 40 shrimp per tank at a salinity of 20. The results showed that the weight gain and specific growth rate were found first increase with the increase in dietary protein levels,and then decrease,the maximal weight gain and specific growth rate in CP40 group,and significantly higher in CP35,CP40 and CP45 groups than those in CP25 group(P＜0.05).Total serum protein,CHOL and TG contents was shown to be increased with increase in dietary protein levels,and then decreased,with the maximum in CP35 group.There were significantly higher total protein contents and CHOL levels in the serum in CP35 and CP45 group than those in CP25 group(P＜0.05),without significant difference in the other groups(P＞0.05).Na+/K+-ATPase activities in the gills were elevated with increase in dietary protein levels and then decreased,the maximum in CP30 and CP35 groups,and significantly higher than those in the other groups(P＜0.05).The quadratic regression between dietary protein levels and growth revealed that the optimal protein requirement was 39.74%for Pacific white leg shrimp at a salinity of 20.
陈思安, 迟淑艳, 梁才强, 谢丽莉, 郭淑珍, 何艳婕, 王文娟. 低盐度下凡纳滨对虾饲料中蛋白质适宜含量的研究[J]. 大连海洋大学学报, 2013, 28(4): 334-339.
CHEN Si-an, CHI Shu-yan, LIANG Cai-qiang, XIE Li-li, GUO Shu-zhen, HE Yan-jie, WANG Wen-juan. The optimal requirement of Pacific white leg shrimp Litopenaeus vannamei for dietary protein at low salinity. Journal of Dalian Ocean University, 2013, 28(4): 334-339.
2013, Vol. 28 
