1.The Center for Applied Aquatic Genomics,Chinese Academy of Fishery Sciences,Beijing 100141,China;2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China
Abstract: Fluorescence in situ hybridization was carried out in chromosomes of scattered mirror carp and Songpu carp(Cyprinus carpio L.)to localize 45S ribosomal DNA(rDNA).Three hybridization probes CAAG1191, CAAG1845,and CAAG3602 were designed for chromosome localization and co-localization from 5.8S rDNA,18S rDNA,and non-transcribed IGS region.The 45S rDNA was found to be localized in only one pair of homologous chromosomes in both common carp.The 45S rDNA derived probes as chromosome specific markers were able to be utilized for chromosome identification in kinds of cytogenetic research for common carp,indicating that the chromosomes related to the 227 cM linkage group 1 in common carp genetic linkage map.Diploid chromosome number of both carp was 2n=100,and no duplication loci were observed in 45S rDNA.The findings suggested that common carp genome was under a re-diploidization process after its whole genome duplication event.
赵紫霞, 邓海霞, 徐鹏, 张研, 李炯棠, 孙效文. 鲤45S rDNA的染色体荧光原位杂交定位[J]. 大连海洋大学学报, 2012, 27(5): 457-463.
ZHAO Zi-xia, DENG Hai-xia, XU Peng, ZHANG Yan, LI Jiong-tang, SUN Xiao-wen#br#. Chromosomal localization of 45S rDNA in common carp by fluorescence in situ hybridization. Journal of Dalian Ocean University, 2012, 27(5): 457-463.
2012, Vol. 27 
