Distribution and histochemical location of four types of intracellular enzymes and mucous cells in the intestines of common carp Cyprinus carpio var. Jian
CHEN Kun, SUN Jingfeng*, HAN Zhuoran, CHEN Limei, HU Xiucai, Lü Aijun
College of Fisheries, Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Tianjin Agricultural University, Tianjin 300384, China
Abstract: The distribution and location of acid phosphatase (ACP), alkaline phosphatase (ALP), peroxidase (POD), non-specific esterase (NSE) and mucous cells were qualitatively and quantitatively determined in the intestine of common carp Cyprinuscarpio var. Jian with body mass of (150±30)g using enzyme histochemical, densitometry technology and statistical analyses. ACP activity was found in the supranuclear region of enterocytes and the lamina propria of the intestine, with the strongest staining in the middle intestine. ALP activity was detected in the striated border and lamina propria of the intestine, with the strongest staining (activity) of ALP in middle intestine and the minimal in the anterior intestine. POD activity was primarily found in the lamina propria and the mucosal muscularis of the intestinal, with the strongest staining in the posterior intestine. The activity of NSE was especially significant in epithelial cells and the staining intensity was strongest in the posterior intestine. Alcian blue (pH 2.5) periodic acid Schiff (AB-PAS) histochemical results revealed that in the intestines there were three types of mucous cells including type Ⅰ, type Ⅱ, and type Ⅲ, which were distributed in the epithelial cells of the mucosa in the anterior intestine, and in the epithelium and lamina propria of the middle intestine and posterior intestine. In conclusion, the activities of ACP, ALP, NSE, and POD and mucous cells were generally distributed in the intestine of common carp, and the characteristics of the enzyme activity and mucous cell distribution were varied with different parts in the intestine.
陈坤, 孙敬锋, 韩卓然, 陈丽梅, 胡秀彩, 吕爱军. 建鲤肠道中4种胞内酶和黏液细胞的分布及组织化学定位[J]. 大连海洋大学学报, 2021, 36(5): 760-766.
CHEN Kun, SUN Jingfeng, HAN Zhuoran, CHEN Limei, HU Xiucai, Lü Aijun. Distribution and histochemical location of four types of intracellular enzymes and mucous cells in the intestines of common carp Cyprinus carpio var. Jian. Journal of Dalian Ocean University, 2021, 36(5): 760-766.
2021, Vol. 36 
