Abstract: In order to study the synergistic stress effects of low salinity and nitrite on “Zebra 2” (shell length 27.5 mm±1.2 mm) and Putian population (shell length 31.2 mm±1.8 mm) of Manila clam (Ruditapesphilippinarum), the salinity of seawater was set at 25, 20 and 15, and the concentration of nitrite nitrogen was set at 0.15, 0.30 and 0.60 mg/L with natural seawater as control at salinity of 30. The oxygen consumption rate and ammonia excretion rate were determined to investigate the effects of low salt and nitrite nitrogen single factor and their synergy on respiratory metabolism of the Manila clam. The results showed that the oxygen consumption rate of the “Zebra 2” and the Putian population increased with the decrease of salinity, and the ammonia excretion rate decreased initially and then increased. With the increasing of nitrite concentration, the oxygen consumption rate of the “Zebra 2” and the Putian population increased, but the ammonia excretion rate of the “Zebra 2” first decreased and then increased. The oxygen consumption rate of the“Zebra 2” and the Putian population increased with the increasing of nitrite concentration under nitrite stress. The ammonia excretion rate of the “Zebra 2” first decreased and then increased, while the ammonia excretion rate of the Putian population first increased and then decreased. Under the synergistic effect of low salinity and high nitrite concentration, the oxygen consumption rate and ammonia discharge rate of the “Zebra 2” and the Putian population increased. Microscopic observation of gill filament structure showed that the gill structure of the Manila clam was damaged, and respiratory epithelial cells fell off under the stress. Through the O∶N analysis of the metabolic process, we found that protein oxidation was the main power of the Manila clam, while, under the synergistic stress of low salt and high nitrite, the main power of the Manila clam was protein, supplemented by fat and carbohydrates. The findings indicate that the synergistic stress of low salt and nitrite affects the normal physiological function of gill tissue of the Manila clam, resulting in the weakening of its respiratory metabolism.
周红, 谷晶, 朱弦一, 郭悦, 杨国军, 霍忠明, 王华. 盐度和亚硝酸盐氮胁迫对菲律宾蛤仔“斑马蛤2号”和莆田群体代谢的影响[J]. 大连海洋大学学报, 2023, 38(4): 630-636.
ZHOU Hong, GU Jing, ZHU Xianyi, GUO Yue, YANG Guojun, HUO Zhongming, WANG Hua. Synergistic stress of salinity and nitrite on metabolism of “Zebra 2” and Putian population of Manila clam (Ruditapes philippinarum). Journal of Dalian Ocean University, 2023, 38(4): 630-636.
2023, Vol. 38 
