Microbial community in intestine of snakehead based on 16S rRNA high-throughput sequencing
XU Shengyun1,2, CHEN Kunci2, LUO Qing2, LIU Haiyang2, OU Mi2, SHANGGUAN Qing1,2, ZHAO Jian2*
1.Key Laboratory of Tropical and Subtropical Aquatic Resources Utilization and Aquaculture, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Abstract: The microbes in intestinal contents were sequenced and analyzed in adult healthy hybrid snakehead Channaargus♀×C.maculate♂ with body weight of 100-150 g collected from Doumen (DM) in Zhuhai, and a pond (BRCT) and cement pond (BRSNC) in Nanhai, and Shunde Xingtan(XT), and wild snakehead with body weitht of 50-100 g collected from Yangjian(YJ) in Foshan by Illumina hiseq 2500 to explore the relationship between the intestinal microorganism and the healthy breeding of snakehead. The results showed that there were some differences in the number of OTUs among the intestinal contents of snakehead in different environments, with the total number of 585, showing that the rich dominant microflora in the contents of the intestinal tract in different environments. Rhodococcus, Clostridium, Microbacterium, Vibrio, Aeromonas, Plesiomonas, Clostridium and Rhodococcus were the dominant flora in all samples, with different proportions in different environments. Heatmap analysis revealed that Proteus, Enterococcus, Lactobacillus, Lactobacillus, Bacteroides, and Corynebacterium were observed intestinal flora, with different proportion, indicating that the number of bacteria was not large. PCA analysis showed that there was the similar intestinal microbial community structure in the artificially-bred hybrid snakehead including DM, BRCT, BRSNC and XT, different from wild YJ. The findings indicated that there were the similar characteristics of microbial community in intestinal contents of snakehead in the same culture environment, and that the composition and diversity of intestinal microflora in different environments were quite different.
张金勇, 高养春, Amir Shah Ruddin Md Sah, Aileen TAN Shau-Hwai, Norlaila Binti Mohd Zanuri, 李宏俊. 基于高通量测序和形态学鉴定的马来西亚马塘红树林区浮游动物多样性分析[J]. 大连海洋大学学报, 2021, 36(6): 910-919.
2020, Vol. 35 
