Abstract: A feeding trial was conducted to study the effects of dietary protein levels(29%,31%,34%,38%,and 40%)on serum biochemical parameters in mirror common carp(Cyprinus specularis)with body weight of 165.24± 8.07 g at different temperature(18,22,and 26℃).The common carp were randomly assigned to 15 groups and fed different diets with triplication and 14 fish in each replicate for 8 weeks.The results showed creatinine (CREA)and creatine phosphokinase(CK)were found to decrease with increase in temperature,significantly lower at 22℃and 26℃than at 18℃(P<0.05).There were no significant differences in triglyceride(TG),lowdensity lipoprotein(LDH),glutamate-oxaloacetate transaminase(AST),and AST/glutamate-pyruvate transaminase(ALT)levels at all temperature(P>0.05),and total cholesterol(CHOL)level was found to decrease significantly at 22℃than at 18℃and 26℃(P<0.05).The blood glucose(GLU),total protein(TP),globulin (GLB),and albumin(ALB)were found to increase with increase in dietary protein levels.At 18℃,LDH level was found to increase as the dietary protein gradually decreased,significantly higher in 38%protein group than that in 29%protein group(P<0.05).At 22℃,CHOL was decreased as protein levels increased,significantly lower in 40%group than that in other protein groups(P<0.05).At 26℃,ALT was significantly higher in 40%group than that in 34%and 38%groups(P<0.05).It was concluded that common carp was considered to live better at 22℃than at 18℃and 26℃according to serum GLU,TP,LDH,CREA,CK,CHOL and ALT levels.The dietary protein of 38%,40%,and 40%was appropriate for serum biochemical parameters in common carp at 18,22,and 26℃.