Characterization of culturable bacterial diversity in the water of Yesso scallop Patinopecten yessoensisis larva rearing tanks
SUN Xue-ying1,LIU Ji-chen1,LIMing2,ZHAO Xue-wei2,LIANG Jun2,SUN Pi-hai3,MA Yue-xin1
1.Key Laboratory ofMariculture and Stock Enhancement in North China蒺s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,Chi-na;2.Zhangzidao Island Group Company Limited,Dalian 116001,China;3.Seafood Seedling Breeding Base,Dalian Ocean University,Dalian 116023,China
Abstract: This study was aimed to examine the culturable bacterial community in the water of larva rearing tanks during Yesso scallop Patinopecten yessoensisis larval development stages(fertilized egg W1,trochophora W2,D-shaped larvaeW3,umbo larvaeW4,and juvenile scallop W5).Thirty strainswere isolated from each water sample by Zobell2216E,and 150 bacterial strainswere identified by polymerase chain reaction and partial16S rRNA gene sequencing.Results showed thatall isolateswere found to be in 2 phyla,3 classes,6 orders,9 families,10 genera and 22 species.At phylum level,Proteobacteria was themost dominant phylum in 5 water samples.At genus lev-el,the first dominantgenus in all sampleswas Pseudoalteromonas,followed by Alteromonas,Vibrio and Marinobact-er atW1 stage,Vibrio,Alteromonas and Cobetia atW2 stage,Vibrio,Alteromonas and Bacillus atW3 stage,Altero-monas,Bacillus and Vibrio atW4 stage,and Vibrio,Bacillus,Pseudophaeobacter and Erythrobacter at W5 stage. The findings show that there are abundant bacterial species and different community structure in the water of larva rearing tanks across different larval development stages.
孙雪莹, 刘继晨, 李明, 赵学伟, 梁峻, 孙丕海, 马悦欣. 虾夷扇贝苗种培育水中可培养细菌群落分析[J]. 大连海洋大学学报, 2017, 32(3): 323-328.
SUN Xue-ying, LIU Ji-chen, LIMing, ZHAO Xue-wei, LIANG Jun, SUN Pi-hai, MA Yue-xin. Characterization of culturable bacterial diversity in the water of Yesso scallop Patinopecten yessoensisis larva rearing tanks. Journal of Dalian Ocean University, 2017, 32(3): 323-328.
2017, Vol. 32 
