Characterization of bacterial community structure on surface of kelp Saccharina japonica by high-throughput sequencing
SUN Pi-hai,QIAN Kun,LI Xiao-li,YANG Zhi-ping,DING Jian-feng,SUN Xue-ying,MA Yue-xin
1.Seafood Seedling Breeding Base,Dalian Ocean University,Dalian 116023,China;2.Dalian Jinzhou District Oceanic and Fishery Administration, Dalian 116100,China;3.Key Laboratory of Mariculture and Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China;4.Dalian Huixin Titanium Equipment Development Company Limited,Dalian 116039,China
Abstract: The genomic DNA was extracted from surface bacteria sampled from healthy kelp Saccharina japonica and surrounding seawater in the sea areas of Dengshahe bay and Zhengmingsi in Dalian in January,February and March of 2015,and a gene segment from the V3-V4 portion of the 16S rRNA gene was sequenced using Illumina Miseq PE300 high-throughput sequencing platform to examine the bacterial community on the surface of the kelp in two sea areas in coastal Dalian.A total of 130 158 and 124 658 optimized sequences were obtained from the kelp and seawater,respectively.Sequences from kelp were classified into 8 phyla and 99 genera,and sequences from seawater were identified as 14 phyla and 135 genera using 16S rRNA gene database.In the kelp community,phylum Firmicutes(＞69%)was predominantly observed,followed by Proteobacteria,Cyanobacteria,Actinobacteria and Bacteroidetes(＞1%).In seawater community,Proteobacteria(＞46%)was most abundant,followed by Firmicutes,Bacteroidetes,Cyanobacteria and Actinobacteria.At genus level,Lactococcus(＞40%)andBacillus(＞10%)were dominant kelp members,followed by Solibacillus,Pseudomonas and Arthrobacter(＞3%),while Vibrio(21.58%)and Lactococcus(28.29%)were the dominant members in seawater from Dengshahe bay and Zhengmingsi,respectively,followed by Colwellia,Arcobacter and Sulfitobacter(＞2%).Dendrogram of hierarchical cluster analysis revealed that there were similar bacterial communities in the two sea areas,and different bacterial communities between the seawater and kelp surface.
孙丕海, 钱坤, 李晓丽, 杨志平, 丁鉴锋, 孙雪莹, 马悦欣. 基于高通量测序分析海带表面细菌群落结构[J]. 大连海洋大学学报, 2017, 32(1): 7-12.
SUN Pi-hai, QIAN Kun, LI Xiao-li, YANG Zhi-ping, DING Jian-feng, SUN Xue-ying, MA Yue-xin. Characterization of bacterial community structure on surface of kelp Saccharina japonica by high-throughput sequencing. Journal of Dalian Ocean University, 2017, 32(1): 7-12.
2017, Vol. 32 
