Influence of some physical and chemical factors on the complement hemolytic activity in sea cucumber Apostichopus japonicus
ZHANG Feng, ZHANG Li
1. School of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China ;2. Key Laboratory of Marine Bio - resources Sustainable Utilization in Liaoning Provinc's University, Dalian Fisheries Univ. , Dalian 116023 ,China
Abstract: The complement hemolytic activity was reported in the coelomic fluid of sea cucumber Apostichopus japonicus, a rate of (68.8 ± 13.5) U/mL. The ACHso assay revealed that the optimum conditions for the complement hemolytic activity in the coelomic fluid were as the following- pH 7.5,8 mmol/L Mg^2+ EGTA - Mg - GVB buffer, at 20 ℃ and reaction period of 90 min. The haemolytic activity was affected by Mg^2+ and inhibited by zymosan, methylamine, hydrazine and PMSF.
张峰, 张莉. 几种理化因子对仿刺参体腔液补体溶血活性的影响[J]. 大连海洋大学学报, 2009, 24(4): 300-304.
ZHANG Feng, ZHANG Li. Influence of some physical and chemical factors on the complement hemolytic activity in sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2009, 24(4): 300-304.