Abstract: The coelomocytes in sea cucumber Apostichopus japonicus were primarily cultured in the sea cucumber coelomic fluid, MEM and L - 15 medium to evaluate the coelomocyte culture conditions. The results showed that the coelomocytes directly cultured in the coelomic fluid showed attachment and extension, with a strongly agglutination under closed culture at 18 ℃, and the cells were shaped like a sphere or skin. The coelomocytes cultured in the compound medium （ MEM, L - 15 ） showed less agglutination. The coelomocytes in L - 15 medium had a relatively long period of attachment and a better stretching fettle, and were cultured in 72 hours compared with MEM medium. There were no significant correlation between the culturing period and cell vitality and the serum concentration.
2009, Vol. 24 
