Abstract: The screening of a K - carrageenase producing bacterium and optimization of fermentation conditions to yield a higher production of extracellular enzyme were conducted by enrichment culture technique and first - screen- ing by K- carrageenan plate containing K- carrageenan as sole source of carbon and energy. A total of 32 isolates that actively degraded K - carrageenan were screened from various seaweeds. Strain HC4 isolated from Hyalosiphonia caespitosa showed the maximum activity by second - screening of shaking culture. Fragment of 1436 bp sequence of strains HC4 16S rRNA gene was amplified, which showed 98% similarity to Tamlana agarivorans when analyzed at National Center for Biotechnology Information （NCBI） database. The culture conditions for the strain HC4 were standardized for the maximal productivity of the extracellular K - carrageenase. The optimal medium components determined by single factor test and orthogonal test were found as the following： K -carrageenan 5 g/L, peptone 3 g/L, NANO3 1 g/L, NaCl 20 g/L, K2HPO4 -3H2O 1 g/L, MgSO4 .7H2O 0.5 g/L, and CaCl2 0.1 g/L. The optimal culture condition were observed as the following： shaking speed 150 r/min, 50 mL medium in 250 mL Erlenmeyer flask, inoculum volume 4%, initial medium pH 7.5, incubation temperature 28 ℃ and incubation time 28 h. In the optimal conditions, the K - carrageenase activity was found to increase by 10. 87 folds compared with the unoptimized conditions.
李俊, 马悦欣, 冯兵, 于杨. 产κ-卡拉胶酶菌株的筛选及其发酵条件的优化[J]. 大连海洋大学学报, 2009, 24(1): 24-29.
LI Jun, MA Yue-xin, FENG Bing, YU Yang. Screening and optimization a K -- carrageenase of fermentation conditions by producing bacterium. Journal of Dalian Ocean University, 2009, 24(1): 24-29.
2009, Vol. 24 
