Abstract: The injection of PHA and Colchicine and chopping method were used for chromo-some preparation from gill, mantle and epipodial tentacles of Pacific ablone Haliotis discus Hannai. The results indicated that the cell mitotic index increased after injection of PHA.The normal metaphase was observed in cells from three kinds of tissue. In this simple method,the mantle and opipodial tentacle were used in vitro for chromesome polyploid breed-ing of ablone.