Effects of dietary Chinese herbal medicine compounds on antioxidant capacity in rainbow trout
SUN Yi-lei, WANG Di , LIU Hong-bai
1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. Fisheries College, Nanjing Agri- cultural University, Nanjing 210095, China
Abstract: A feeding trial was conducted to evaluate the effects of two kinds of Chinese herbal compound on antioxidant defenses of rainbow trout Oncorhynchus mykiss for application of Chinese herbal compound in rainbow trout culture. Juvenile rainbow trout of 1+?old with body weight of (200.0±1.5)g were randomly divided into outdoor circulating circular tanks at a rate of 120 fish per tank and fed basal diet and the diets containing two kinds of Chinese herbal medicine compounds (No.1 Artemmisia capillaries and Isatis indigotica; No.2 Arnebia guttata and Lonicera japonic)at feeding rate of 2.5% with duplication at water temperature of (17±1) ℃ for 30 days. The NO and malonaldehyde (MDA) contents, activities of total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC) were determined in serum, liver, spleen and kidney of the rainbow trout fasted for 24 hours at the end of the experiment. The results showed that there were significantly higher activity of SOD and significantly lower content of MDA in liver in the rainbow trout fed the diets containing Chinese herbal medicines than those in the juveniles fed the basal diet (P<0.05). The contents of NO in liver, spleen and kidney, the activity of iNOS in spleen, and the serum T-AOC were found to be increased significantly in the rainbow trout fed the diets containing Chinese herbal compound 1 (P<0.05), with higher activity of GSH-PX in serum. The rainbow trout fed the diets containing Chinese herbal compound 2 had significantly higher content of NO in the kidney. The findings indicate that compound 1 improves the antioxidant capacity effectively, and can be used as an excellent antioxidant of Chinese herbal medicine for rainbow trout.
孙裔雷, 王荻, 刘红柏. 复方中草药对虹鳟抗氧化能力的影响[J]. 大连海洋大学学报, 2015, 30(2): 170-174.
SUN Yi-lei, WANG Di , LIU Hong-bai. Effects of dietary Chinese herbal medicine compounds on antioxidant capacity in rainbow trout. Journal of Dalian Ocean University, 2015, 30(2): 170-174.