Functional effects of pma-m iR-200c-3p of sea lam prey on heart development of zebrafish
LIU Yun1，WANG Cong-cong1，2，GUO Ya-nan1，XU Qiang-hua1，2，3，4
1.College of Marine Sciences，ShanghaiOcean University，Shanghai201306，China；2.Key Laboratory of Sustainable Exploitation ofOcean Fisheries Resources，Ministry of Education，Shanghai 201306，China；3.National Distant-water Fisheries Engineering Research Center，Shanghai 201306，China；4.Collaborative Innovation Center for Distant-water Fisheries，Shanghai201306，China
Abstract: The pma-miR-200c-3p mimics from sea lamprey Petromyzon marinus were injected into zebrafish Danio rerio embryos bymicroinjection，and qRT-PCR and Western blotwere used to analyze themarker gene expression of the development of heart，and the expression levels of several genes in BMP/Smad pathway to study the biological function of pma-miR-200c-3p of the sea lamprey.Besides，target gene bio-informatics and GFP fluorescence expression analysiswere used to study the function of pma-miR-200c-3p.The results showed that the gene expression levels of some cardiac developmentmarkers including bmp4，bmp5，smad1，gata5，tbx2b and notch1a were significantly increased in the pma-miR-200c-3p-injected embryos（P＜0.01）.The protein expression levels of the BMP4，Smad1 and GATA5 were significantly increased bya BMP/Smad pathway，and it appears that cdx1b is a targetgene of pma-miR-200c-3p.Therefore，the findings indicate that pma-miR-200c-3pmight repress the gene expression of cdx1b，and be involved in the BMP/Smadpathway to promote the cardiac development process. Key words：Petromyzon marinus；miR-200c-3p；zebrafish；cardiac development；cdx1b
发布日期: 2016-12-30
期的出版日期: 2016-10-21
中图分类号:
Q951
引用本文:
刘云, 王丛丛, 郭亚南, 许强华. 海七鳃鳗pma-miR-200 c-3 p对斑马鱼心脏发育的作用研究[J]. 大连海洋大学学报, 2016, 31(5): 510-515.
LIU Yun, WANG Cong-cong, GUO Ya-nan, XU Qiang-hua, . Functional effects of pma-m iR-200c-3p of sea lam prey on heart development of zebrafish. Journal of Dalian Ocean University, 2016, 31(5): 510-515.
2016, Vol. 31 
