Transmissibility characteristics of methomyl in chlorella-water fleas-zebrafish food chain
CHEN Feng, MENG Shunlong*, CHEN Jiazhang*, QIU Liping, FAN Limin, SONG Chao, ZHENG Yao, LI Dandan, HU Gengdong
1.National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 2.Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract: To probe into the bioaccumulation and transfer the effect of methomyl in aquatic organisms, green alga Chlorellavulgaris was exposed to 0, 2, 10, and 50 μg/L of methomyl for 4 days, and fed to water fleas Daphniamagna for 9 days which then fed to zebrafish Daniorerio for 9 days at water temperature of (20±1)℃. The results showed that there was bioaccumulation factor (BCF) as high as 17 549.99, and the bioaccumulation amount of 33.28 μg/g in the green alga exposed to the low concentration groups (2 μg/L) methomyl for 72 h. In the high concentration feeding groups (50 μg/L)， the water fleas fed the green alga exposed to methomyl for 4 days had the maximum bioaccumulation amount of 6.02 μg/g， and biomagnification factor (BMF) of 0.177 0 for 9 days without biomagnification effect. The maximal methomyl concentration of 0.72 μg/g was observed in the body of zebrafish exposed to 50 μg/L methomyl directly for 4 days (exposure group). The green alga had a strong ability to accumulate methomyl, significantly higher than the water fleas (0.12 μg/g)(P<0.05). Methomyl was passed to the water fleas through the feeding process， and then passed to the zebrafish by the water fleas through the feeding process. The passed methomyl inhibited life activities such as food intake and metabolism.
2022, Vol. 37 
