Cloning of atp6v0c gene from Antarctic notothenioid fish Dissostichus mawsoni and identification of its cold tolerance in HeLa cell
LIU Ming-li，WANG Jin-feng，ZHANG Dong，CHEN Liang-biao
College of Fisheries and Life，Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources，Ministry of Education，Shanghai Ocean University，Shanghai 201306，China
Abstract: The atp6v0c gene of Antaratic notothenioid fish Dissostichus mawsoni was cloned from Antarctic notothenioid fish cDNA library，and then transfected the dmatp6v0c gene obtained into the HeLa cell to detect cell viability under cold stress（10℃）by flow cytometry to investigate the ATPase responsive for the cold adaptation in Antarctic notothenioid fish.The complete coding sequence with 462 bp of dmatp6v0c gene was cloned and sequenced，and the dmatp6v0c coding sequence was inserted into the pcDNA3.1（-）vector by XhoI and BamHI restriction sites for gene expression in HeLa cell.The results showed that over-expression of dmatp6v0c gene in HeLa cell was found to reduce the cell mortality from 19.23%±1.87%to 8.13%±0.04% （P＜0.05）under cold stress compared with the control group.The findings indicated that the expression level of dmatp6v0c was heavily involved in cell viability under cold stress and that dmatp6v0c could be considered as a candidate for breeding cold resistant fish.
刘明丽, 王金凤, 张东, 陈良标. 鳞头犬牙南极鱼atp6 v0 c基因在HeLa细胞中抗寒功能的研究[J]. 大连海洋大学学报, 2016, 31(1): 7-12.
LIU Ming-li, WANG Jin-feng, ZHANG Dong, CHEN Liang-biao. Cloning of atp6v0c gene from Antarctic notothenioid fish Dissostichus mawsoni and identification of its cold tolerance in HeLa cell. Journal of Dalian Ocean University, 2016, 31(1): 7-12.
2016, Vol. 31 
